sox19b在斑马鱼胚胎眼睛发育中的作用

doi:10.3969/j.issn.0529-1356.2013.02.023

解剖学报 ›› 2013, Vol. 44 ›› Issue (2 ): 264-268.doi: 10.3969/j.issn.0529-1356.2013.02.023

• 组织学胚胎学发育生物学 • 上一篇下一篇

sox19b在斑马鱼胚胎眼睛发育中的作用

高明杨阳陈学冉史玮林浴霜郝爱军*

山东大学医学院组织学胚胎学教研室，教育部实验畸形学重点实验室，济南250012

收稿日期:2012-09-12 修回日期:2012-11-01 出版日期:2013-04-06 发布日期:2013-04-06
通讯作者: 郝爱军 E-mail:aijunhao@sdu.edu.cn

Effect of sox19b gene knockdown on the eyes development in zebrafish

GAO Ming YANG Yang CHEN Xue-ran SHI Wei LIN Yu-shuang, HAO Ai-jun*

Key Laboratory of the Ministry of Education for Experimental Teratology, Department of Histology and Embryology,School of Medicine, Shandong University, Ji’nan 250012, China

Received:2012-09-12 Revised:2012-11-01 Online:2013-04-06 Published:2013-04-06

摘要/Abstract

摘要：

目的通过抑制sox19b基因的表达，探讨sox19b基因在斑马鱼胚胎眼睛发育和形成中的作用。方法通过显微注射sox19b 吗啉寡聚核苷酸（MO）抑制sox19b 基因的表达，观察胚胎发育过程中表型的变化；采用石蜡包埋组织切片及HE染色、RT-PCR和整封原位杂交等方法探讨敲除sox19b 后对斑马鱼胚胎眼睛发育的调控机制。结果敲除sox19b 基因后，斑马鱼胚胎眼睛发育受到影响，表现为眼睛变小或缺失，视网膜及晶状体结构发育异常(n =57/93)；眼睛发育过程中重要调控基因 rx3、pax2a及 vsx2 等表达明显降低，进而影响眼睛的发育和形成。结论 sox19b 基因作为转录调控因子，可以通过调节眼区转录因子的表达进而影响斑马鱼胚胎早期眼睛的发育和形成。

关键词: sox19b , 吗啉寡聚核苷酸 , 眼睛发育 , 眼区转录因子 , 原位杂交 , 反转录-聚合酶链反应 , 斑马鱼

Abstract:

Objective To explore the function of sox19b on the eye development in zebrafish by knockdown of sox19b. Methods To inhibit the expression of sox19b by microinjection of sox19b morpholinos（MO）and then observe the effect of the development of the eyes in zebrafish by RT-PCR and the whole-mount in situhybridization. Results The development of the eyes was abnormal after knockdown of sox19b, mainly showed small eyes or eye deficiency and the abnormal retina and lens(n =57/93). The expression of some important genes during the development of the eyes, such as rx3, pax2a and vsx2, was decreased apparently in the sox 19b morphant. Conclusion The transcription factor, sox19b is critical for the development of the eyes via the regulation of the important eye-field transcription factors,EFTEs in early zebrafish embryos.

Key words: Sox19b , Morpholinos , Development of the eyes , Eye-field transcription factor , In situ hybridization , RT-PCR , Zebrafish

中图分类号:

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高明杨阳陈学冉史玮林浴霜郝爱军* . sox19b在斑马鱼胚胎眼睛发育中的作用[J]. 解剖学报, 2013, 44(2 ): 264-268.

GAO Ming YANG Yang CHEN Xue-ran SHI Wei LIN Yu-shuang, HAO Ai-jun*. Effect of sox19b gene knockdown on the eyes development in zebrafish[J]. AAS, 2013, 44(2 ): 264-268.

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sox19b在斑马鱼胚胎眼睛发育中的作用

Effect of sox19b gene knockdown on the eyes development in zebrafish

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